Establishing the first DNA barcode reference library for freshwater decapod species (Crustacea: Decapoda) in Republic of North Macedonia

The freshwater ecosystems in the Republic of North Macedonia are considered as biodiversity hotspot on European level since they provide diverse habitats that correspond with complex ecological preferences many species require. This specially applies to crustaceans that, unfortunately, have never been focus a continuous research. In R. Macedonia, usually inhabit exposed negative anthropogenic impact. Thus, some may become extinct presenting an irreversible loss for Macedonian natural heritage. Although DNA barcoding, highly effective tool fast detection, is already routine protocol taxonomical studies all over world, there still no official national barcoding initiative Macedonia. study employs based ca. 650-bp long standard fragment mt COI gene Astacus astacus , Austropotamobius torrentium Potamon fluviatile and ibericum previously identified morphological characters, collected from different parts ability rapidly identify has proven. Our research presents first comprehensive molecular decapod taxonomy giving attempt establish barcode reference library country. We hope further application this approach will lead construction aquatic biota Such find purpose modern bioassessment protocols well phylogenetic detecting interpopulation genetic variability.